A physical sciences network characterization of non-tumorigenic and metastatic cells.

TitleA physical sciences network characterization of non-tumorigenic and metastatic cells.
Publication TypeJournal Article
Year of Publication2013
AuthorsAgus DB, Alexander JF, Arap W, Ashili S, Aslan JE, Austin RH, Backman V, Bethel KJ, Bonneau R, Chen W-C, Chen-Tanyolac C, Choi NC, Curley SA, Dallas M, Damania D, Davies PCW, Decuzzi P, Dickinson L, Estevez-Salmeron L, Estrella V, Ferrari M, Fischbach C, Foo J, Fraley SI, Frantz C, Fuhrmann A, Gascard P, Gatenby RA, Geng Y, Gerecht S, Gillies RJ, Godin B, Grady WM, Greenfield A, Hemphill C, Hempstead BL, Hielscher A, W Hillis D, Holland EC, Ibrahim-Hashim A, Jacks T, Johnson RH, Joo A, Katz JE, Kelbauskas L, Kesselman C, King MR, Konstantopoulos K, Kraning-Rush CM, Kuhn P, Kung K, Kwee B, Lakins JN, Lambert G, Liao D, Licht JD, Liphardt JT, Liu L, Lloyd MC, Lyubimova A, Mallick P, Marko J, McCarty OJT, Meldrum DR, Michor F, Mumenthaler SM, Nandakumar V, O'Halloran TV, Oh S, Pasqualini R, Paszek MJ, Philips KG, Poultney CS, Rana K, Reinhart-King CA, Ros R, Semenza GL, Senechal P, Shuler ML, Srinivasan S, Staunton JR, Stypula Y, Subramanian H, Tlsty TD, Tormoen GW, Tseng Y, van Oudenaarden A, Verbridge SS, Wan JC, Weaver VM, Widom J, Will C, Wirtz D, Wojtkowiak J, Wu P-H
Corporate AuthorsPhysical Sciences - Oncology Centers Network
JournalSci Rep
Volume3
Pagination1449
Date Published2013
ISSN2045-2322
KeywordsCell Line, Tumor, Cell Movement, Cell Size, Cell Survival, Computer Simulation, Gene Expression Regulation, Neoplastic, Humans, Models, Biological, Neoplasm Metastasis, Neoplasm Proteins, Tumor Markers, Biological
Abstract

To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

DOI10.1038/srep01449
Alternate JournalSci Rep
PubMed ID23618955
PubMed Central IDPMC3636513
Grant ListK12 GM081266 / GM / NIGMS NIH HHS / United States
R01 CA077575 / CA / NCI NIH HHS / United States
R01 CA138818 / CA / NCI NIH HHS / United States
R01 CA165309 / CA / NCI NIH HHS / United States
U01 ES019458 / ES / NIEHS NIH HHS / United States
U54 CA143836 / CA / NCI NIH HHS / United States
U54 CA143837 / CA / NCI NIH HHS / United States
U54 CA143869 / CA / NCI NIH HHS / United States
U54CA143798 / CA / NCI NIH HHS / United States
U54CA143803 / CA / NCI NIH HHS / United States
U54CA143836 / CA / NCI NIH HHS / United States
U54CA143837 / CA / NCI NIH HHS / United States
U54CA143862 / CA / NCI NIH HHS / United States
U54CA143868 / CA / NCI NIH HHS / United States
U54CA143869 / CA / NCI NIH HHS / United States
U54CA143874 / CA / NCI NIH HHS / United States
U54CA143876 / CA / NCI NIH HHS / United States
U54CA143906 / CA / NCI NIH HHS / United States
U54CA143907 / CA / NCI NIH HHS / United States
U54CA143970 / CA / NCI NIH HHS / United States