Scanning angle interference microscopy reveals cell dynamics at the nanoscale.

TitleScanning angle interference microscopy reveals cell dynamics at the nanoscale.
Publication TypeJournal Article
Year of Publication2012
AuthorsPaszek MJ, DuFort CC, Rubashkin MG, Davidson MW, Thorn KS, Liphardt JT, Weaver VM
JournalNat Methods
Volume9
Issue8
Pagination825-7
Date Published2012 Aug
ISSN1548-7105
KeywordsCell Membrane, Cells, Cultured, Cytoskeleton, Epithelial Cells, Fibronectins, Focal Adhesions, Humans, Microscopy, Interference, Nanotechnology
Abstract

Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes.

DOI10.1038/nmeth.2077
Alternate JournalNat. Methods
PubMed ID22751201
PubMed Central IDPMC3454456
Grant List1U54CA163155-01 / CA / NCI NIH HHS / United States
R01 CA138818 / CA / NCI NIH HHS / United States
R01 CA138818-01A1 / CA / NCI NIH HHS / United States
U54 CA143836 / CA / NCI NIH HHS / United States
U54 CA163155 / CA / NCI NIH HHS / United States
U54CA143836-01 / CA / NCI NIH HHS / United States